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Genetic variation between two Verticillium dahliae type strains using rapd analysis [electronic resource].

By:

Farrag, Rasha Mohamed

Language: English Summary language: Arabic Description: P.85-94Other title:

التنوع الجيني بين سلالتين من نوع الفرتيسليوم دالياى باستخدام تقنية التضخيم العشوائي عديد الأوجة للحامض النووى دأوكسى ريبوز [Added title page title]

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Annals of agricultural science, 2007 v. 52 (1) [electronic resource]

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In: Annals of Agricultural Science 2007.v.52(1)Summary: The genetic variation between two Verticillium dahliae type strains was investigated. The two investigated type strains were different in their colony characteristics; V. dahliae CBS 179.66 possesses white colonies reflecting a defect in microsclerotia formation while V. dahliae var. longisporum CBS 649.85 has dark colonies ensuring the production of microsclerotia. RAPD analyses, using four primers, revealed their great intraspecific variations where a large number of polymorphic bands were generated. RFLP-ITS revealed their interspecific relationship. This study is a basis for a number of molecular research concerning microsclerotia which are the highly resistant and viable structures responsible for the perennation of V. dahliae spp. in soil.

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The genetic variation between two Verticillium dahliae type strains was investigated. The two investigated type strains were different in their colony characteristics; V. dahliae CBS 179.66 possesses white colonies reflecting a defect in microsclerotia formation while V. dahliae var. longisporum CBS 649.85 has dark colonies ensuring the production of microsclerotia. RAPD analyses, using four primers, revealed their great intraspecific variations where a large number of polymorphic bands were generated. RFLP-ITS revealed their interspecific relationship. This study is a basis for a number of molecular research concerning microsclerotia which are the highly resistant and viable structures responsible for the perennation of V. dahliae spp. in soil.

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