Molecular and phytochemical characterization of some Silybum landraces grown in Egypt [electronic resource].

Includes references.

A total of 20 random primers were evaluated for their ability to prime PCR amplification of 12 Silybum genotypes in a preliminary survey using genomic DNA of plants from each genotype. RAPD analysis revealed 128 scorable. bands from two primers. including 36 (28.125%) polymorphic bands. The band pattern revealed differences between the collected genotypes. Certain band changes were found in Romanin genotype plants and between Egyptian genotype, suggesting the existence of genetic variation which might .affect the biochemical synthesis, of the different genotypes tested in this Study. The results of HPLC analyses for the different genotypes. revealed that the highest content of siIychristin. Silydianin, Silybinin (A, B) and total silymarin were detected in samples collected from Romanian genotype cultivated in Egypt. Desert road of Alexandria genotype, Kafr EI-Sheikh genotype and Bani-sewaf genoty)pe, respectively. the lowest content of silychristin, Silydianin. Silybinin (A, B) and total silymarin were found in samples, collected from Desert road of Alexandria genotype where .siIychristin was absent, .agricultural road of Alexandria. genotype where Silydianin was absent, desert road of Alexandria genotype and Romania genotype. respectively. The results of this study confirmed that the difference in geographical locations and the genetic, variation between silybum genotypes have a great effect in their RAPD fingerprints and contents of silymarin.

1