Transfer of phosphate dissolving ability of B. megatherium var phosphaticumto B. polymyxa via genetic transformation [electronic resource].

Includes references.

The present investigation was carried out to transfer the phosphate dissolving ability of B. mega/herium var phospha/icum to B. polymyxa via genetic transformation. B. polymyxa was used as recipient strain and Bacillus megatherium as a donor. Transformation was detected on phosphate dissolvers media. Transformation frequency was found to be 3.15x10⁻³ • Seven transformants were selected and tested for their phosphate dissolving and N,-fixing abilities on liquid media (ill vitro) and on wheat plants grown in sterilized soil (ill vitro). The obtained results indicated that all transformants exhibited pbosphate dissolving ability, but transformants No. 5 was the most efficient one. However, transformation did not affect N,-fixing ability of Bacillus.

Summary in Arabic.

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