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Cryosurvivng and fertilizing abilities of rahmany ram spermatozoa after freezing-thawing using untraditional packaging methods [electronic resource].

By:

Awad, M.M

Contributor(s):

Khalifa, E. I

Language: English Summary language: Arabic Description: p.113-118Uniform titles:

Agricultural research journal, Suez Canal University, 2010 v.10(1) [electronic resource].

Subject(s):

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In: Agricultural Research Journal, Suez Canal University 2010 v.10(1)Summary: The most successful packaging techniques for frozen ram spermatozoa are pellets using dry ice and straws but there are economical and commercial pressures to use other methods of packaging techniques in developing countries. Semen was collected of the experiment using an artificial vagina. Ejaculates, from three different rams, containing greater than 70% progressive motility were pooled together and considered to be one sperm sample. Forty eight ewes were used in this study to test the fertilizing ability of ram spermatozoa. The thawed semen was assessed for total motility (TM), and morphology of spermatozoa characteristics (MSC). TM and MSC were assessed after thawing and 0, 2, 4 and 6 h of incubation at 37°C. The morphology of spermatozoa (dead sperm, abnormal tails and damaged head) was assessed by staining frozen-thawed spermatozoa with eosin-nigrosin dye. Fourteen sperm samples were extended, frozen, thawed and analyzed.

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The most successful packaging techniques for frozen ram spermatozoa are pellets using dry ice and straws but there are economical and commercial pressures to use other methods of packaging techniques in developing countries. Semen was collected of the experiment using an artificial vagina. Ejaculates, from three different rams, containing greater than 70% progressive motility were pooled together and considered to be one sperm sample. Forty eight ewes were used in this study to test the fertilizing ability of ram spermatozoa. The thawed semen was assessed for total motility (TM), and morphology of spermatozoa characteristics (MSC). TM and MSC were assessed after thawing and 0, 2, 4 and 6 h of incubation at 37°C. The morphology of spermatozoa (dead sperm, abnormal tails and damaged head) was assessed by staining frozen-thawed spermatozoa with eosin-nigrosin dye. Fourteen sperm samples were extended, frozen, thawed and analyzed.

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