Isozyme patterns of esterase in larvae of the wax moth, Galleria Mellotlella L. infected with Bacillus thuringiensis kurstaki [electronic resource].

Includes references.

Activity of esterase in larvae of the greater wax moth, Galleria mellonella L. infected with LC50 of Dipel 2X based on Bacillus thuringiensis kurstaki; (4.784 g mixed i 1 100 g diet) was studied by PAGE. The isozyme electrophoretic patterns showed 59 peptides in both healthy and treated larvae. Based on their relative mobility, 17 peptides of high mobility (1.99 kda) appeared in both larval groups among the 6 days of the test; from which 7 peptides were specific to the healthy larvae ,i.e., P1, P5; P6; P8; P11; P12 and P17 with molecular weights of 1, 6, 15, 38, 53, 62 and 99 kda, respectively: and 9 peptides were found specific for diseased larvae, i,e., P2 (3kda), P3 (4 kda), P4 (5 kda), P7 (]7 kda), P9 (51 kda), PI] (78 kda), PI4 (91 kdal, PIS (92 kda), PIG (93 kda) and PIG (93 kda).Meanwhile, the peptide PlO (52 kda) was non-specific and appeared in both healthy and diseased Larvae. Isoenzyme patterns of moderate mobility (101-194 kda) recorded 30 peptides; 10 specific for healthy, 17 for treated larvae. and 3 nonspecific On the other hand. 12 peplides of low mobility (211-315 kda) were recorded: most of them (8 peptides) being specific in healthy larvae and 2 specific for infected larvae. while 2 appeared non-specific. Clustering of the obtained peptides formed a dendrogram showing their similarity % that correlates the new peptides in the B. t. kurstaki treated larvae to the action of delta endotoxin on the activity of the esterase enzyme.

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