A modified method to determine hydrogen peroxide activity as a quality criterion of bee boney [electronic resource].

Includes references.

Honey contains glucose oxidase which converts glucose to gluconic acid and H2O2 This enzyme is active in the unripe or dilutee honey. Produced H2O2 is unstable and is decomposed to oxygen and water Adding a chromogen, in the presence of peroxidase. oxygen oxidizes this chromogen and changes it from colorless to be colored. In the present study. accumulation of H2O2 (as an activity measure of glucose oxidase) in honey was monitored in the presence of leucocrystal violet. Three common Egyptian honeys from different floral sources; citrus. clover, and cotton produced during Three successive seasons were tested. The obtained results showed lhat fresh honeys exhibited higher enzyme activity than those stored for two or one year, respectively. Cotton honey had the higher enzyme and H2O2 activities followed by clover honey. while citrus honey was lhe least active one. It was observed lhat dilution of honey increased enzyme activity gradually wilh increase in dilution rate. simultaneously the time needed for reaching a maximum concentration of H2O2 was extended. From lhese results it could be concluded that this melhod could be used to judging honey quality and distinguishing those stored or adulterate.

Summary in Arabic.

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