Normal view MARC view ISBD view

Some serological and immunological studies on Oestrus ovis infesting sheeph[electronic resource].

By:

Omar, S.F.A

Contributor(s):

Al-Sukayran, A

Language: English Summary language: Arabic Description: p.251-263Other title:

بعض دراسات السيرولوجية والمناعية على الدودة النغفية فى الأغنام [Added title page title]

Uniform titles:

Assiut veterinary medical journal, 2006 v. 52 (109) [electronic resource].

Subject(s):

Online resources:

Full Text Article.

In: Assiut Veterinary Medical Journal 2006.v.52(109)Summary: Four hundred and twenty sheep were examined for Oestrus ovis larval infestation by naked eye and serological test (Direct ELISA) during six months (from June to November, 2005). Excretory - secretory product (ESP) from first (L1) and second (L2) instar of O.ovis larvae were used as a coating antigen and a vaccine. The naked eye examination showed a positive prevalence of 19.5 % while the ELISA test showed 24.3 %. The obtained data proved that the sensitivity of ELISA test was 97.6 %. Twenty female lambs of three months old were allocated into two groups. Tne first one received two 1M injections of ESP in the neck, 4 weeks apart, initially in Freunds complete adjuvant and then in Freunds incomplete adjuvant. In control group, the animals received two injections of PBS with Freunds complete and incomplete adjuvants. Challenge test was carried out twice, IS (15 larvae per animal) and 28 days (25 larvae per animal) after the second immunization. Sera samples were collected for ten weeks.

Average rating: 0.0 (0 votes)

No physical items for this record

Includes references.

Four hundred and twenty sheep were examined for Oestrus ovis larval infestation by naked eye and serological test (Direct ELISA) during six months (from June to November, 2005). Excretory - secretory product (ESP) from first (L1) and second (L2) instar of O.ovis larvae were used as a coating antigen and a vaccine. The naked eye examination showed a positive prevalence of 19.5 % while the ELISA test showed 24.3 %. The obtained data proved that the sensitivity of ELISA test was 97.6 %. Twenty female lambs of three months old were allocated into two groups. Tne first one received two 1M injections of ESP in the neck, 4 weeks apart, initially in Freunds complete adjuvant and then in Freunds incomplete adjuvant. In control group, the animals received two injections of PBS with Freunds complete and incomplete adjuvants. Challenge test was carried out twice, IS (15 larvae per animal) and 28 days (25 larvae per animal) after the second immunization. Sera samples were collected for ten weeks.

Summary in Arabic.

There are no comments on this title.

to post a comment.