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Cryopreservation of in vitro established shoot tip explants of date palm cv. zaghlool [electronic resource].

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El-Dawayati, Miaada M

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Language: English Summary language: Arabic Description: p.477-497Other title:

الحفظ بالتجميد للمنفصلات النباتية المجهزة للقمم النامية لنخيل البلح (صنف الزغلول) [Added title page title]

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Egyptian journal of agricultural research, 2007 v. 85 (1) B, Special issue [electronic resource]:

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In: 2007.v.85(1)B,SPSummary: Cryoprotection procedures for in vitro established shoot tip explants of Date palm cv. Zaghlool prior to cryoprservation process in liquid nitrogen were investigated in this study. This work aimed to develop simple cryoprotection procedure that can be handled easily. The first cryoprotection procedure was to study the effects of different sucrose concentrations (0.1, 0.3, 0.5, and 0.7Mol) in preculturing medium and the incubation under at 5°C or 2rC for four weeks. The second cryoprotection procedure was to study the effects of the same previous sucrose concentrations in preculturing medium and the incubation under at 2rC for four weeks followed by desiccation procedure for two hours in an open petri dish exposed to continual current stirel air in the laminar airflow cabint.

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Cryoprotection procedures for in vitro established shoot tip explants of Date palm cv. Zaghlool prior to cryoprservation process in liquid nitrogen were investigated in this study. This work aimed to develop simple cryoprotection procedure that can be handled easily. The first cryoprotection procedure was to study the effects of different sucrose concentrations (0.1, 0.3, 0.5, and 0.7Mol) in preculturing medium and the incubation under at 5°C or 2rC for four weeks. The second cryoprotection procedure was to study the effects of the same previous sucrose concentrations in preculturing medium and the incubation under at 2rC for four weeks followed by desiccation procedure for two hours in an open petri dish exposed to continual current stirel air in the laminar airflow cabint.

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