Isolation and sequencing of insulin-like growth factor 1 (IGF-1) from Egyptian buffalo via RT-PCR [electronic resource].

Includes references.

Insulin-like growth factor 1 (IGF 1) is a member of a heterogeneous group of peptides with important growth-promoting effects in vitro as well as in vivo. It plays a fundamental role in postnatal mammalian growth as a major mediator through which growth hormone exerts its biological effects. We report the isolation and sequencing of full-length IGF-1 cDNA from bovine liver, encoding the IGF-1 protein. RT-PCR reaction was performed in which gene-specific primers corresponding to IGF-1 were utilised employing Pfu, a proof reading and high-fidelity thermostable DNA polymerase. A PCR product of 543 bp, including the open reading frame of IGF-1 gene corresponding to the expected theoretical product size, was successfully amplified. Sequencing of the purified PCR product and its double alignment against a previously known bovine gene identified it as a cDNA corresponding to IGF-1 with the open reading frame encoding the entire IGF-1 precursor of 154 amino acids.

Summary in Arabic.

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