Role of selective antioxidants and anti-inflammatory drug in preventing udder cell damage in vitro [electronic resource].

Includes references.

The current study aimed to investigate the role of melatonin, carotene, selenium, and hydrocortisone in protecting a primary mammary cell line, as a model of mastitis, from the deleterious effects of reactive oxygen species (ROS) secreted from activated neutrophils in vitro. Different concentrations of melatonin (0.5,5,50, and 500 µM), carotene (1, 10, and 100 µM), selenium (0.001, 0.01, and 0.1 µM), and hydrocortisone (0.1, I, and 10 µM) were added separately in triplicates to primary mammary epithelial cell lines prepared from active mammary tissue of female buffalo co-incubated with activated buffalo neutrophils (5 X 10/well). Mammary cell damage was evaluated by measuring lactate dehydrogenase (LDH), malondialdehyde (MDA) and nitric oxide (NO) concentrations in culture media as indicative of ROS, and by morphological observations of neutrophils after acridine orange staining.

Summary in Arabic.