Effects of oocyte quality, maturation media and gonadotropins on invitro maturation of buffalo oocyte [electronic resource].

Includes references.

This study was carried out to investigate the effect of oocyte quality and different culture media as well as hormones on in vitro maturation of buffalo oocytes. A total of 1197 oocytes were collected from 591 ovaries obtained from a slaughterhouse. The number of follicles on ovarian surface was counted and the number of follicles / ovary was calculated. Oocytes were aspirated and the number of recovered oocytes per ovary was calculated. The recovery rate was determined. Oocytes were classified according to the appearance of cumulus cells into excellent, good, fair and poor. They were cultured either in TCM-199 or mSOF with different hormonal supplements (FSH, hCG or combination of both) for 24 h in CO2 incubator at 38°C. Assessment of maturation by cumulus cell expansion and nuclear maturation was carried out. The results revealed that 174 out of 258 excellent oocytes reached G3 with a percentage of 67.4%, 143 out of 249 good oocytes reached G3 with 57.4%. and 146 out of309 fair oocytes reached G3 with 47.2% while all 280 poor (denuded) oocytes did not reach G3. Excellent oocytes exhibited higher significant (P<O.Ol) value than the other qualities.

Summary in Arabic.