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Relationship between O-Serogroup, virulence and plasmids profile in E.coli isolated from diseased chickens [electronic resource].

By:

Enany, Mohammad. E

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Language: English Summary language: Arabic Description: P. 58-65Other title:

العلاقة بين التصنيف السيرولوجي و الضراوه و صورة البلازميد في ميكروب الاشيريشياكولاى المعزوله من الدجاج المريض [Added title page title]

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Zagazig veterinary journal, 2008 v. 36 (1) [electronic resource].

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In: Zagazig Veterinary Journal 2008.v.36(1)Summary: A total of 197 E.coli strains were isolated from diseased chickens from different farms in Ismailia Governorate. The serotyped strains (n=40) were belonged to 10 serovars, 078(13), 0153(6), 0168(5), 026(4),0157 and 0146(3/each), 020 and 0114(21each), 0125 and 0126(l/each). The results showed that 95% of E.coli serovars were Congo red positive, while 70% produced haemolysin. All investigated serovars produced cytotoxins for Vero cells (verocytotoxigenic). PCR assay was carried out for six serovars to detect the presence of eae A gene, only one serovar had intimin gene (0125:H7). The plasmids profile of the most important E.coli serovars were examined revealing that all contained plasmids. It was concluded that there was an association between plasmids containing strains and their high virulence.

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A total of 197 E.coli strains were isolated from diseased chickens from different farms in Ismailia Governorate. The serotyped strains (n=40) were belonged to 10 serovars, 078(13), 0153(6), 0168(5), 026(4),0157 and 0146(3/each), 020 and 0114(21each), 0125 and 0126(l/each). The results showed that 95% of E.coli serovars were Congo red positive, while 70% produced haemolysin. All investigated serovars produced cytotoxins for Vero cells (verocytotoxigenic). PCR assay was carried out for six serovars to detect the presence of eae A gene, only one serovar had intimin gene (0125:H7). The plasmids profile of the most important E.coli serovars were examined revealing that all contained plasmids. It was concluded that there was an association between plasmids containing strains and their high virulence.

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