Effect of methyl B cyclodextrin on freezability and in vitro fertilizing potential of buffalo spermatozoa [electronic resource].

Includes references.

Cryopreservation induces partially irreversible damage to sperm membrane as a result of membrane destabilization. The membrane damage after freezing might be linked to the modification of the membrane fluidity before freezing. Methyl f3 cyclodextrin (mf3CD) was found to selectively extract cholesterol from the plasma membrane and therefore, increased membrane fluidity. The present study aimed at investigating the effect of adding mf3CD to the Tris-based diluent on the freezability and in vitro fertilizing potentials of buffalo spermatozoa. Buffalo semen was collected, evaluated and extended in Tris-based extender supplemented with different concentrations of mf3CD (experiment 1) or with cholesterol-3-sulfate and/or mf3CD (experiment2). Semen was examined post-thawing to evaluate their freezability, cholesterol content and in vitro fertilizing potentials. The present results revealed that, addition of mf3CD to the semen diluent before freezing improved significantly the freezability and fertilizing potentials in a dose dependent trend.

Summary in Arabic.