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Cryopreservation of immature buffalo oocytes by vitrification [electronic resource].

By:

Badr, M. R

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Language: English Summary language: Arabic Description: p.397-412Other title:

تجميد بويضات الجاموس الغير ناضجة بالتزجج [Added title page title]

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Assiut veterinary medical journal, 2008 v. 54 (117) [electronic resource].

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In: Assiut Veterinary Medical Journal 2008.v.54(117)Summary: Cryopreservation of collected oocytes from slaughtered animals of high genetic value, for production of embryos may provide an opportunity to replenish the valuable germplasm lost. The aim of this study was to cryopreserve the immature buffalo oocytes by vitrification. Experiments were conducted to study the effect of using different ethylene glycol concentrations (10, 20 and 40%) in the equilibration solution, equilibration periods (3, S and 10 min) and vitrification solutions (ethylene glycol with 0.5 M sucrose:, 0.3 M trehalose and 20% dimethyl sulfoxide) with a non vitrified group served as control on morphological survival, in vitro maturation and embryo development of vitrified-warmed immature buffalo oocytes. The selected cumulus oocyte complexes with compacted cumulus cells and evenly granulated ooplasm were vitrified.

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Cryopreservation of collected oocytes from slaughtered animals of high genetic value, for production of embryos may provide an opportunity to replenish the valuable germplasm lost. The aim of this study was to cryopreserve the immature buffalo oocytes by vitrification. Experiments were conducted to study the effect of using different ethylene glycol concentrations (10, 20 and 40%) in the equilibration solution, equilibration periods (3, S and 10 min) and vitrification solutions (ethylene glycol with 0.5 M sucrose:, 0.3 M trehalose and 20% dimethyl sulfoxide) with a non vitrified group served as control on morphological survival, in vitro maturation and embryo development of vitrified-warmed immature buffalo oocytes. The selected cumulus oocyte complexes with compacted cumulus cells and evenly granulated ooplasm were vitrified.

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