Detection and isolation of rota (group a) and corona (group 2) viruses from nomadic camels with special reference to pathological alterations [electronic resource].

Includes references.

To study the presence of rota (group A) and corona (group 2) viruses in camels, a total of 229 fecal samples were collected from diarrheic (130) as well as contact diarrheic( with other animal species) (64) and healthy camels (35) from different nomadic flocks from desert areas in Sharkia Governorate, Egypt during 2006- 2007. Group A rota virus infection was confirmed by direct fluorescent anti-body technique (FAT), virus isolation and detection of rota virus antigen by immune-captured enzyme linked immune sorbent assay (Ic-ELISA) in feces and harvested inoculated cell, as well as demonstration of cytopathic effect (CPE) and haemadsorbtion criteria of rota virus on Vera cell. Corona virus antigen was detected by FAT, Ic-ELISA but all attempts for corona virus isolation were entirely failed. Specific rota virus or corona virus antigens or both; were detected by Ic-ELISA; in 44.01% (1011229), 23.14% (53/229) and 10.91% (25/229) of the total examined fecal specimens, respectively, of which 53.07% (69/130),34.61% (45/130) and 17.69% (23/130) were from diarrheic, 34.37% (22/64), 12.50% (8/64) and 3.12% (2/64) from contact animals for rota, corona or both virus antigens, respectively and 28.57% (10/35), for rota virus only from healthy camels. The results show that the rota virus is widespread, mainly among camels from 6 to 12-months old, while corona virus alone or mixed with rota virus was 1 to 2 years old. The histopathological changes in small intestine associated with rota virus infection were observed in villus atrophy and flattening of the epithelial cells. The small and large intestines infected by corona virus had multifocal mucosal ulceration and various degrees of mucosal to submucosal edema with moderate accumulation of lymphocytes. These findings emphasis the significant role of rota virus and corona virus or both in the enteric infection of camels in Egypt.

Summary in Arabic.