Analysis of genetic interaction between CRY2 gene and floral meristem identity and actin polymerization pathways in Arabidopsis thaliana L. Heynh [electronic resource].

Includes references.

This work was carried out to detecat the genetic interaction between Arabidopsis blue light photoreceptor cryptochrome 2 (CRY2) gene and the gene of the floral meristem identity pathway. The influence of Arabidopsis Actin Related Protein complex (ATARP2/3 complex) on flowering initiation and the kind of interaction with CRY2 gene was also studied and evaluated. Several parameters such as flowring time, E. coli β-glucuronidase gene (GUS) activity assay, RNA and protein expression were used. Several monogenic and double mutanyt lines have been prepared and planted in different photoperiod coditions. Data analysis showed indirect genetic interaction (additive) between CRY2 gene and the floral meristem identity pathway. Despit the negative regulation effect of DIS2 gene in the floral meristem identity pathway and GRL in the ARP2/3 complex on flowering time, there was on direct genetic iteraction between CRY2 gene and the ARP2/3 complex. In future studies, work will be devoled towards the detection of a possible direct genetic interaction between ATARP2/3 complex and the different flowering pathway.

Summary in Arabic.

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