In vitro regeneration of sugarbeet propagules and molecular analysis of the regenerants [electronic resource].

Includes references.

An applicable regeneration protocol through in vitro direct and indirect organogenesis of sugarbeet was developed. Leaf and shoot base explants taken from in vitro grown seedlings were subjected to a medium containing different combinations of cytokinins (BA and Kin) and auxins (NAA and 2,4-D). The highest percentages of direct organogenesis were noticed with shoot base explants; however, leaf explant was more readily for indirect differentiation. Concerning growth regulators added to culture media, cytokinins (BA and kin) in combinations with NAA were more effective on direct regeneration since the best results of shoot emergence directly from shoot base explants (93%) were registered with a medium contained 0.5 mg/l BA + 0.5 mg/l NAA. However, the highest frequency of indirect regeneration (80%) was obtained when leaf explants were cultured on a medium containing 0.5 mg/l kin + 0.5 mg/l 2,4-D.

Summary in Arabic.

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