Normal view MARC view ISBD view

Expression of the bovine coronavirus spike glycoprotein subunits in insect cells using recombinant baculoviruses [electronic resource].

By:

Amer, H. M

Contributor(s):

Language: English Summary language: Arabic Description: p.385-398Other title:

إنتاج الجزيئان التركيبيان للبروتين السطحي(إس)لفيروس الكورونا البقري بواسطة فيروسات الباكولو المدمجة في الخلايا الحشرية [Added title page title]

Uniform titles:

Arab journal of biotechnology, 2007 v. 10 (2) [electronic resource]:

Subject(s):

Online resources:

Full Text Article

In: Arab journal of biotechnology 2007.v.10(2)Summary: In the present study, the bovine coronavirus (BCV) spike glycoprotein cleavage products (S1 and S2) were individually expressed in Spodoptera frugiperda (Sf9) insect cells, using a baculovirus expression system. The coding sequence of S1 and S2 gene fragments were amplified by RT-PCR and cloned into the baculovirus shuttle vector pBlueBac4.5/V5-His TOPO ® TA. The cloned fragments were inserted into the genome of Autographa californica nuclear polyhydrosis virus (AcMNPV) under control of the polyhedrin promoter, through a process of homologous recombination between the shuttle vector and a linearized replication-defective baculovirus DNA (Bac-N-Blue™).

Average rating: 0.0 (0 votes)

No physical items for this record

Includes references.

In the present study, the bovine coronavirus (BCV) spike glycoprotein cleavage products (S1 and S2) were individually expressed in Spodoptera frugiperda (Sf9) insect cells, using a baculovirus expression system. The coding sequence of S1 and S2 gene fragments were amplified by RT-PCR and cloned into the baculovirus shuttle vector pBlueBac4.5/V5-His TOPO ® TA. The cloned fragments were inserted into the genome of Autographa californica nuclear polyhydrosis virus (AcMNPV) under control of the polyhedrin promoter, through a process of homologous recombination between the shuttle vector and a linearized replication-defective baculovirus DNA (Bac-N-Blue™).

Summary in Arabic.

There are no comments on this title.

to post a comment.