Plantlet regeneration from callus of Cilrullus colocynthis collected from El-Wadi El-Gadid region as a medicinal plant [electronic resource].

By: Contributor(s): Language: English Summary language: Arabic Description: p.783-791Other title:
  • استيلاد نباتات من كالوس نبات حنظل الوادي الجديد كنبات طبي [Added title page title]
Uniform titles:
  • Egyptian journal of plant breeding, 2007 v. 11 (2) Special issue [electronic resource]:
Subject(s): Online resources: In: Egyptian Journal of Plant Breeding 2007.v.11(2).Special issue.Part-2Summary: Regeneration in Citrullus colocynthis collected from EL- Wadi EI-Gadid region as a medicinal plant has been achieved through the disorganized culture (callus). Results silo wedl that tile best explants fur calius furmation were; the coryledun and hypocoryledon when, cultured on MS+ 0..1 mg/L kinetin + different concentrations of 2, 4-D + 0.8 % agar + 3 " sucrose at pH 5.8. This medium induced a golden callus at 20 'C and 1000 lux for 8 hr/16 hr light /dark period. The best medium for plantlet regeneration from callus was MS + 0.1 mg/ L 1AA + different concentrations of 6 BA + 0.8 % agar + 3 % sucrose at pH (5.7) under conditions of 30c. 1500 lux and 10 hr light / Uhr dark period. It was shown that maximum regeneration capacity for one callus was 59 R. planets. Rooting medium consisted of % MS + different concentrations of NAA + 0.7 % agar + 3 % sucrose at pH (5.7). The in vitro regeneration of this plant would prevent its eradication due to expansion of housing in its area of natural growth.
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Regeneration in Citrullus colocynthis collected from EL- Wadi EI-Gadid region as a medicinal plant has been achieved through the disorganized culture (callus). Results silo wedl that tile best explants fur calius furmation were; the coryledun and hypocoryledon when, cultured on MS+ 0..1 mg/L kinetin + different concentrations of 2, 4-D + 0.8 % agar + 3 " sucrose at pH 5.8. This medium induced a golden callus at 20 'C and 1000 lux for 8 hr/16 hr light /dark period. The best medium for plantlet regeneration from callus was MS + 0.1 mg/ L 1AA + different concentrations of 6 BA + 0.8 % agar + 3 % sucrose at pH (5.7) under conditions of 30c. 1500 lux and 10 hr light / Uhr dark period. It was shown that maximum regeneration capacity for one callus was 59 R. planets. Rooting medium consisted of % MS + different concentrations of NAA + 0.7 % agar + 3 % sucrose at pH (5.7). The in vitro regeneration of this plant would prevent its eradication due to expansion of housing in its area of natural growth.

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