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Molecular characterization of Corynebacterium pseudotuberculosis isolated from sheep by random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) [electronic resource].

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Gooda, Azza S. A

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Language: English Summary language: Arabic Description: p.39-49Other title:

الخصائص الجزيئية لعترات كوريني السل الكاذب المعزول من الأغنام باستخدام التمديد العشوائي لأجزاءالحمض النووي [Added title page title]

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Beni-Suef veterinary medical journal, 2007 v. 17 (1) [electronic resource].

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In: Beni-Suef Veterinary Medical Journal 2007.v.17(1)Summary: The phenogenetic and phylogenetic relationships among 7 identified strains of C. pseudotuberculosis isolated from sheep were checked by PCR specific primers demonstrated with random amplified polymorphic DNA – polymerase chain reaction (RAPD – PCR) by using five arbitrarily primers. C pseudotuberculosis isolates were distinguished according to the banding patterns of their amplified DNA on agarose gel. The variation can be used for diagnostic differentiation among C. pseudotuberculosis strains. Differences were observed in RAPD patterns between the 7 isolates and this may be due to the presence of novel serovars of this microorganism.

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The phenogenetic and phylogenetic relationships among 7 identified strains of C. pseudotuberculosis isolated from sheep were checked by PCR specific primers demonstrated with random amplified polymorphic DNA – polymerase chain reaction (RAPD – PCR) by using five arbitrarily primers. C pseudotuberculosis isolates were distinguished according to the banding patterns of their amplified DNA on agarose gel. The variation can be used for diagnostic differentiation among C. pseudotuberculosis strains. Differences were observed in RAPD patterns between the 7 isolates and this may be due to the presence of novel serovars of this microorganism.

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