Improvement of plant regeneration from long-term callus cultures of two Egyptian wheat cultivars [electronic resource].

Includes references.

An efficient protocol was developed for regeneration of plants from long-term cultured calluses, originated from immature embryos of two Egyptian wheat (Triticum aestivum L.) cultivars (Sids 1 and Giza 168). For callus induction and long-term maintenance, calluses were subcultured onto Murashige and Skoog's medium containing 2.0 mg/l 2, 4-D for eight months. The calli were then cultured onto a regeneration medium supplemented with Zeatin ribozide (0, 0.5 and 1.0 mg/l) or Thidiazuron (0, 0.1 and 0.2 mg/l) or Dicamba (0, 0.25 and 0.5 mg/l). Among the two cultivars, Sids1 produced the highest number of shoots per shooted callus (4.5) in TDZ containing medium (0.2mg/l). Important differences in regeneration characteristics were observed between the two genotypes. The developed in vitro system for maintaining of embryogenic calli for prolonged period is essential for conducting mutation, selection and genetic modifications for improvement of wheat cultivars.

Summary in Arabic.

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