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RAPD and SDS-PAGE analysis of R4 salinity-tolerant wheat (Triticum Aestivuml.) regenerant lines [electronic resource].

By:

Abdel-fatah, O. M

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Language: English Summary language: Arabic Description: p.2219-2233Other title:

التكبير العشوائى المتعدد الصور للحمض النووى دنا والتفريد الكهربى لبروتين سلالات القمح المتحملة للملوحة المستولدة من الجيل الرابع [Added title page title]

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Egyptian journal of agricultural research, 2007 v. 85 (6) [electronic resource].

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In: Egyptian Journal of Agricultural Research 2007.v.85(6)Summary: The objective. of this study was to examine the banding pattern of four salinity tolerant and four sensitive regenerated (R.4) wheat genotypes. PCR-RAPD Randomly Amplified Polymorphic DNA technique as well as SDS-(PAGE) Sodium dodecyl sulfate polyacrylamide gel electrophoresis were applied on H lines (High¬salinity tolerant lines) and L lines (Low-salinity tolerant lines) chosen from R4 wheat Triticum aestivum L. regenerate lines together with their parents (Sakha 8, Lines 25 and 28) under control and salinity stress conditions to determine any associated molecular markers with salinity tolerance/susceptibility genes.

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The objective. of this study was to examine the banding pattern of four salinity tolerant and four sensitive regenerated (R.4) wheat genotypes. PCR-RAPD Randomly Amplified Polymorphic DNA technique as well as SDS-(PAGE) Sodium dodecyl sulfate polyacrylamide gel electrophoresis were applied on H lines (High¬salinity tolerant lines) and L lines (Low-salinity tolerant lines) chosen from R4 wheat Triticum aestivum L. regenerate lines together with their parents (Sakha 8, Lines 25 and 28) under control and salinity stress conditions to determine any associated molecular markers with salinity tolerance/susceptibility genes.

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