Quantity and quality of harvested buffalo oocytes in relation to ovarian structures and methods of collection [electronic resource].

Includes references.

A total number of 68 buffalo-cows ovaries were collected from slaughterhouse. In the first part of this study, 40 ovaries were classified according to the ovarian structures into: those with dominant follicle (DF, n = 14) , with corpus luteum (CL, n=9 ), with DF and CL (n=5) and those without DF or CL (no dominant structure: nDS, n = 12). Surface foll1cles between 3 and 8 mm were enumerated and aspirated The aspirated follicular fluids were examined microscopically for the oocytes. The recovered oocytes were counted and evaluated according to number and quality of the cumulus layers and character of the ooplasm. In the second part of the study, the obtained oocytes were compared with those obtained by slicing technique applied on 28 ovaries. The results revealed that, the average number of aspirated follicles/ovary was higher from ovaries with nDS (7.33 ± 3.8), than from ovaries with DF (3.67±2.2), p<0.5. Also, the average number of recovered oocytes/ovary was higher from ovaries with nDS (5.75:i:: 2.4), than from those with DF (2.79±2), p<0.05. Ovaries which carried CL showed in-between results (4.0:1:3.7 aspirated follicles and 3.22:1:3.1 recovered oocytes). Higher number of oocytes with more than 5 cumulus layers was collected from ovaries with nDS (28.9%) than from ovaries with DF (5.1%), p<0.05. Slicing technique increased significantly the average number of recovered oocytes/ovary than the aspiration technique, (5.00 ± 2.3 vs 3.90±2.4) and the number of oocytes with more than 5 cumulus layers (62.1 % vs 15.3%) p<0.05. It is concluded that 1) DF adversely affected the number and quality of recovered oocytes in buffalo, 2) ovarian slicing technique increased significantly the quantity and quality of the harvested oocytes.

Summary in Arabic.