Isolation and identification of Edwardsiella Tarda from infected Nile Tilapia fish "Oreochromis Niloticus" [electronic resource].

Includes references.

Edwardsiella tarda (E. tarda) is one of the important warm water-fish pathogens causing serious economic loss. This work was planned to isolate and identify pathogenic E. tarda from naturally infected Nile tilapia fish, Oreochromis niloticus by different methods. Out of 200 randomly collected fish, fifty naturally displayed the clinical symptoms of E. tarda infection. Seven strains, isolated from the diseased fish, were selected according to their colony characteristics. Biochemical results of these seven isolates gave the pattern of the bacterium E. tarda. Antibiotic sensitivity, against ten antibiotics, confirmed identifying all the seven isolates as identical E. tarda. The incidence of that pathogen in the liver, kidneys and spleen was 28.57% for each, while 14.29% were isolated from the intestine, whereas the deep musculature was free of E. tarda. Experimental infection, by intramuscular injection, exhibited typical signs of Edwardsiellosis on all injected fish and 57.5% mortality within two weeks.

Summary in Arabic.

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