Infectious clones of red clover necrotic mosaic virus RNA transcribed in vitro from cDNA amplified with the polymerase chain reaction [electronic resource].

Includes references.

Full-length cDNA to RNA1 of red clover necrotic mosaic virus strain TpM-34. (RCNMV-TpM34) was amplified using the polymerase chain reaction (PCR). The first strand primer contained a NotI site and sequences complementary to the 3'-terminus of the RNA1 of the RCNMV Australian strain. The second-strand primers contained a NotI site, T7 promoter and a sequence corresponding to the 5'-terminus of RNAI of the RCNMV Australian strain. After cleavage with NotI, the PCR products were cloned into the NotI site of the vector pUCBM20. Five clones were selected and RNA transcripts were synthesized in vitro from each clone using T7 RNA polymerase. Two transcripts were found to be infectious when inoculated into cowpea plants in conjunction with RNA2 transcripts.

Summary in Arabic.

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