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Use of isozyme composition of seed to quantity resistace of flax cultivars to powdery mildew disease [electronic resource].

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Aly, A. A

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Language: English Summary language: Arabic Description: p.3425-3438Other title:

استعمال محتوى البذرة من المشابهات اانزيمية للتعبير الكمي عن مقاومة أصناف الكتان لمرض البياض الدقيقي.‪ [Added title page title]

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Mansoura University journal of agricultural sciences, 2005 v. 30 (6) [electronic resource].

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In: Mansoura University Journal of Agricultural Sciences 2005.v.30(6)Summary: A field trial was conducted in 200212003 and 2003/2004 growing seasons at Giza Agricultural Research Station to evaluate the reactions of ten flax cultivars to powdery mildew (PM) disease. In general, the tested cultivars could be divided into four distinct groups, i.e., highly resistant (Ottowa 770 B, Dakota, and Bombay), resistant (Cass, Wilden, and Clay), susceptible (Koto and Marshall), and highly susceptible (Cortland and C.\. 2008). The cultivars showed considerable variation in disease severity (OS) ranged from 3.69 on Bombay to 100% on C.\. 2008. Isozymes of malate dehydrogenase (MOH), peroxidase (PRX) and esterase (EST) of cultivar seed were separated by polyacrylamide gel electrophoresis (PAGE), and the obtained banding patterns were visualized by using three staining systems. Data for PM severity (dependant variable), and amounts of isozymes (independent variables or predictors) were entered into a computerized stepwise multiple regression analysis. Using the predictors supplied by stepwise regression2 three models were constructed to quantify PM severity. Coefficient of determination (R ) values of the models were 98.67, 83.46, and 83.46% for MOH, PRX and EST, respectively. These results indicate that PAGE of isozymes may provide a supplementary assay to greenhouse and field tests to distinguish quantitatively between PM resistant or susceptible cultivars.

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A field trial was conducted in 200212003 and 2003/2004 growing seasons at Giza Agricultural Research Station to evaluate the reactions of ten flax cultivars to powdery mildew (PM) disease. In general, the tested cultivars could be divided into four distinct groups, i.e., highly resistant (Ottowa 770 B, Dakota, and Bombay), resistant (Cass, Wilden, and Clay), susceptible (Koto and Marshall), and highly susceptible (Cortland and C.\. 2008). The cultivars showed considerable variation in disease severity (OS) ranged from 3.69 on Bombay to 100% on C.\. 2008. Isozymes of malate dehydrogenase (MOH), peroxidase (PRX) and esterase (EST) of cultivar seed were separated by polyacrylamide gel electrophoresis (PAGE), and the obtained banding patterns were visualized by using three staining systems. Data for PM severity (dependant variable), and amounts of isozymes (independent variables or predictors) were entered into a computerized stepwise multiple regression analysis. Using the predictors supplied by stepwise regression2 three models were constructed to quantify PM severity. Coefficient of determination (R ) values of the models were 98.67, 83.46, and 83.46% for MOH, PRX and EST, respectively. These results indicate that PAGE of isozymes may provide a supplementary assay to greenhouse and field tests to distinguish quantitatively between PM resistant or susceptible cultivars.

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