Genotyping identification of locally isolated Eimeria species used in evaluation of imported chicken coccidiosis vaccines [electronic resource].

Includes references.

A multiplex polymerase chain reaction (PCR) assay was used for the detection of the circulating Eimeria species in the domestic chicken flocks in 2 Egyptian governorates. Three types of intestinal samples were collected from freshly dead birds diagnosed to have coccidial infestation. These samples were pooled from duodenal. cecal and the remained intestinal contents separately. The oocysts of each sample were washed, purified and concentrated. DNA was extracted from each sample and utilized in a multiplex PCR assay using 7 pairs of species-specific primers. Eimeria acervulina (E. acervulina) and E. praecox were detected in the pooled duodenal samples. only E. tennella was detected in the cecal samples, and the known seven Eimeria species were detected in the mixed intestinal samples. The results of this work assure the infestation of the Egyptian chicken flocks with all the known seven Eimeria species.

Summary in Arabic.

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