Clinicopathological and immunological studies on Toxoid vaccine as a successful alternative in controlling clostridial infection in broilers [electronic resource]

Includes references.

Three vaccination regimes based on Clostridium perfringens (C perfringens) type A, C or combined AC· toxoids were evaluated to detect if toxoid vaccines caD prevent necrtotic enteritis (NE) caused by clostridial infection in broilers. The vaccines were administeredJwo times at two weeks iDterva~ then the birds were challenged with virulent straios of C. per/Tingen! type A, C or combined AC.: Blood samples were taken after both first and second vaccination doses as well as after challengi Evaluating parameters included clinical signs, gross intestinal lesions, hemogram [red blood ced count (RBCs), packed ·cell volume (PCV), hemoglobin (Hb), total leukocytic count (TLC) and differential leukocytic countl,serum biochemical assays [total protein (TP), albumin (Alb), globulin (Glob), albumin globulin ratio (AlG), serum activities of alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP) and uric acid (UA) values) and ELISA test for detecting serum antibody titers. Results revealed that affected birds showed marked depression, anorexia, reluctance to move, rumed feathers and diarrhea. Numbers oC chickens with intestinal lesions in immunized c~allenged groups were greatly fewer than the infected non immunized ones. There was an increase in RBCs, rcv and Mh. TLC decreased in infected non immunized birds and increased in vaccinated ones. Heterophils were increased in infected gronps while lymphocytes decreased.

Summary in Arabic.

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