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Differentiation between virulent and avirulent strains of Pasteurella multocida using restriction endonuclease analysis [electronic resource].

By:

Salama, S. S

Contributor(s):

EI-Ebiary, Elham A

Language: English Summary language: Arabic Description: p.177-179Uniform titles:

Beni-Suef veterinary medical journal, 2005 v. 15 (2), Special issue [electronic resource]:

Subject(s):

Online resources:

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In: Beni-Suef Veterinary Medical Journal. Beni-Suef Vetrinary Medicen Journal November 2005, 4th Scientific conference 2005.v.15(2)(Special issue)Summary: In this study we used one reference Climson University (CU) strain and 3 virulent field strains isolated from naturally infected chicken and identified as P. Multocida. DNA was extracted from all strains and subjected to restriction endonuclease analysis, using EcoRI, Hpall and Hind III revealing that, great similarity between either the reference or local virulent field isolates. The obtained results indicated that the most differentiable restriction endonuclease enzyme was the Hind III, which showed different band patterns between different strains.

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In this study we used one reference Climson University (CU) strain and 3 virulent field strains isolated from naturally infected chicken and identified as P. Multocida. DNA was extracted from all strains and subjected to restriction endonuclease analysis, using EcoRI, Hpall and Hind III revealing that, great similarity between either the reference or local virulent field isolates. The obtained results indicated that the most differentiable restriction endonuclease enzyme was the Hind III, which showed different band patterns between different strains.

Summary in Arabic.

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