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Preparation of diagnostic Lysate antigens for rapid evaluation of local entero-3 vaccine [electronic resource].

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EI-Kholy, A. A

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Language: English Summary language: Arabic Description: p.184-189Uniform titles:

Beni-Suef veterinary medical journal, 2005 v. 15 (2), Special issue [electronic resource]:

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In: Beni-Suef Veterinary Medical Journal. Beni-Suef Vetrinary Medicen Journal November 2005, 4th Scientific conference 2005.v.15(2)(Special issue)Summary: Studies were conducted to determine the utility of lysate antigens for rapid evaluation of the local entero-3 vaccine, antigens were prepared from cell cultures infected with bovine rota virus (BRV) and bovine corona virus (BCV) as well as from Enterotoxigenic E. coli strain K99. Prepared antigens were tested with field serum samples collected from both late pregnant entero-3 vaccinated cows and their off springs using different serological assays including: microagglutination test, indirect ELISA and immunonuorescent antibody technique. Results of this endeavor were correlated to that of the standard virus neutralization test. The locally prepared antigens were proved useful for vaccine evaluation. Moreover, these antigens are recommended for both detection and assessment post vaccination or post infection of sero-conversion against BRV, BCV arid E. coli.

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Studies were conducted to determine the utility of lysate antigens for rapid evaluation of the local entero-3 vaccine, antigens were prepared from cell cultures infected with bovine rota virus (BRV) and bovine corona virus (BCV) as well as from Enterotoxigenic E. coli strain K99. Prepared antigens were tested with field serum samples collected from both late pregnant entero-3 vaccinated cows and their off springs using different serological assays including: microagglutination test, indirect ELISA and immunonuorescent antibody technique. Results of this endeavor were correlated to that of the standard virus neutralization test. The locally prepared antigens were proved useful for vaccine evaluation. Moreover, these antigens are recommended for both detection and assessment post vaccination or post infection of sero-conversion against BRV, BCV arid E. coli.

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