Direct regeneration of Egyptian cottons (Gossypium barbadense L) from cotyledonary nodes and shoot apex [electronic resource].

Includes references.

Plant transgenic technology is an attractive means for introduction of foreign genes into cotton, but it requires an effective plant regeneration system. This study was aimed to develop a high frequency shoot regeneration technique from cotyledonary nodes and shoot apex of Egyptian Cottons (Gossypium barbadense L.). Shoot proliferation from different explants of seven Egyptian cotton varieties namely: Giza 45, Giza 70, Giza 83, Giza 86, Giza 88, Giza 89 and Giza 90 was studied in culture. The proliferation was carried out on B5 Gamberg medium supplemented with different cytokinins (6 combinations). The results indicated that the addition of cytokinins in the medium induced the number of produced shoots compared to the control, however differences based on the cytokinin type and concentration and nature of the explants were observed. Cotyledonary nodes produced higher number of shoots compared to shoot apex of the same vareity.

Summary in Arabic.

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