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Immunological studies on the local infectious bursal disease virus (IBD) adapted on specific pathogen free embryonated chicken eggs SPF (ECE) [electronic resource].

By:

Brahim, Nadia M. I

Language: English Summary language: Arabic Description: P. 709-729Other title:

دراسة القدرة المناعية للعترة المعزولة محليا لفيروس التهاب غدة فابريشيس بعد تمريرها على آجنة البيض الخالى من المسببات المرضية [Added title page title]

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Egyptian journal of agricultural research, 2011 v. 89 (2) [electronic resource].

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In: Egyptian Journal of Agricultural Research 2011.v.89(2)Summary: Local isolate of (IBD) virus [EID50 106/ml] was propagated for three passages on (SPF) embryonated chicken eggs (EIDso 106.s/ml) followed by three passages on SPF chickens (EID50 10 8/ml), then, for hundred and ten serial passages on SPF embryonated chicken eggs prepared and titrated (EIDso 107/ml) after every 5 passages. The virus was inoculated into 21 days old chickens and observed for 21 days post-infection, then, challenged with virulent IBD virus. Passages (110, 77, 55) were used for preparation of live attenuated vaccines mild, intermediate, hot (IBDV) vaccines on SPF (ECE), respectively. The evaluation of the prepared vaccines was carried out for sterility, safety and potency. The potency was performed by measuring humoral and cellular immune response, as well as, protection percentage against virulent IBDV. The Efficacy of the prepared vaccine was estimated for up to six months.

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Local isolate of (IBD) virus [EID50 106/ml] was propagated for three passages on (SPF) embryonated chicken eggs (EIDso 106.s/ml) followed by three passages on SPF chickens (EID50 10 8/ml), then, for hundred and ten serial passages on SPF embryonated chicken eggs prepared and titrated (EIDso 107/ml) after every 5 passages. The virus was inoculated into 21 days old chickens and observed for 21 days post-infection, then, challenged with virulent IBD virus. Passages (110, 77, 55) were used for preparation of live attenuated vaccines mild, intermediate, hot (IBDV) vaccines on SPF (ECE), respectively. The evaluation of the prepared vaccines was carried out for sterility, safety and potency. The potency was performed by measuring humoral and cellular immune response, as well as, protection percentage against virulent IBDV. The Efficacy of the prepared vaccine was estimated for up to six months.

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