Microsatellites as markers for Egyptian wheat genotyping [electronic resource].

Includes references.

Microsatellite markers have been shown to be highly polymorphic and simple to we in hexaploid wheat. This study was undertaken with the following objectives: (1) to assess the level of polymorphism among 19 Egyptian wheat varieties and (1) to study the potential of microsatellite markers and specify microsatellite(s) in particular for Egyptian wheat genotype identifications A total of 41 microsatellite primers were included which, were located on all 21 chromosome pairs with distribution of one microsatellite marker per chromosome-arm of A. B and D genomes. The total number of alleles detected was 171 and the number amplified by each locus ranged from 1 to 10 with an average of 4.10 alleles. Calculated polymorphic information content (PIC) values ranged from 0.11 to 0.86 with 30 loci (71. 43%) scoring 0.50 or higher and the average was 0.57. In the present study, only seven primers of the 42 could discriminate the 19 Egyptian wheat genotypes. Therefore, the present set of the 42 microsatellite primers would be useful for detecting polymorphism and for DNA fingerprinting of Egyptian wheat genotypes. Finally, this set of microsatellite markers could act as a reference set of markers for a range of research application for Egyptian wheat.

Summary in Arabic.

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