Field Evaluation for Leaf Rust Disease and Rapd Analysis for Somaclonal Variant Lines in Wheat [electronic resource].

Includes references.

Somaclones , derived from five local commercial wheat cultivars , were evaluated for resistance to leaf rust under greenhouse conditions. The results indicated that the ratio of the resistance to susceptibility varied, based on the somaclones, as well as virulence of the pathotypes. The analysis of variance for the degrees of rust severity (%) in leaf rust revealed significant differences among the two wheat cultivars (Giza-164 and Sakha-69) and their somaclones. However, the differences between the other two cultivars ( Gemmiza-1 and Giza-167 ) and their somaclones were not significant. As for field evaluation, statistical analysis showed that the number of grains per spike and grain yield per plant recorded significant differrences among wheat cultivars and their somaclones, except for the cultivar,Gemmiza-1. It, also, revealed that there were differences among Gemmiza-1 and their somaclones, as well as Giza-167 and their somaclones in flowering date, but the mean number of spikes per plant revealed significant differences only among Sakha-69 and their somaclones. The results obtained from RAPD analysis for wheat cultivars and their somaclones revealed that six primers were used to amplify DNA segments from the genomic DNA of the four wheat cultivars, Giza-164, Sakha-69, Gemmiza-1 and Giza-167, and their somaclones. Forty-nine amplification products were obtained, all of these amplified fragments were polymorphic .The RAPD markers, produced by six primers, were used to construct a similarity matrix . The genetic similarity among the fourteen genotypes ranged from 0.05 to 0.89. The wheat cultivars and their somaclones were classified into seven clusters .

Summary in Arabic.

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