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Genetically resistant cucumber plants to wilt pathogen via tissue cultures [electronic resource].

By:

El-Kazzaz, A. A

Contributor(s):

Ashour, A. M. A

Language: English Summary language: Arabic Description: p.1-10Other title:

نباتات خيار مقاومة وراثيا لمسبب الذبول باستخدام المزارع النسيجية [Added title page title]

Uniform titles:

Egyptian journal of phytopathology, 2004 v. 32 (1-2) [electronic resource].

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Full Text Article.

In: Egyptian Journal of Phytopathology 2004.v.32(1-2)Summary: Resistant calli were in vitro selected from cucumber explants under challenging stress of cucumber wilt pathogen Fusarium oxysporum f.sp. cucumerinum culture filtrates (CF). The selection protocol has two directions: First one is step-by-step selection from lower to higher selective CF concentrations; meanwhile the second one is exchangeable continuous cycles with and/or without CF using the same selective CF concentration until the end of selection regime. The progenies of in vitro regenerated plants, occurred under CF stress, showed resistance when exposed to the pathogen infection. The results cleared that resistance in cucumber to wilt pathogen is controlled by one pair of genes and segregated as 3 resistant : 1 susceptible. That in vitro selective regime via tissue cultures is advisable for selection of novel disease-resistant plants. Key words: Biotechnology, callus, disease-resistance plants, genetics, plant protection, somaclonal variations and tissue cultures.

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Resistant calli were in vitro selected from cucumber explants under challenging stress of cucumber wilt pathogen Fusarium oxysporum f.sp. cucumerinum culture filtrates (CF). The selection protocol has two directions: First one is step-by-step selection from lower to higher selective CF concentrations; meanwhile the second one is exchangeable continuous cycles with and/or without CF using the same selective CF concentration until the end of selection regime. The progenies of in vitro regenerated plants, occurred under CF stress, showed resistance when exposed to the pathogen infection. The results cleared that resistance in cucumber to wilt pathogen is controlled by one pair of genes and segregated as 3 resistant : 1 susceptible. That in vitro selective regime via tissue cultures is advisable for selection of novel disease-resistant plants. Key words: Biotechnology, callus, disease-resistance plants, genetics, plant protection, somaclonal variations and tissue cultures.

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