Molecular phylogenetic relationships of two genera of Labiatae family [electronic resource].

Includes references.

Leaves of seven genotypes of Labiatae family represented two genera; four species of Mentha genus (M. Viridis, M. Piperita, M. Arvensis and M. longifolia) and three species of genus Ocimum (O. basilicum, O. canum and O. basilicum Marseille) were collected to identify molecular markers for each and to study the similarity indices and phylogenetic relatinships among these species. Two PCR- based analysis systems were carried out using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR). RAPD-PCR analysis using 21 random primers successfully exhibited a total of 399 DNA fragments across the seven genotypes, with 100 species-specific markers. Similarity indices ranged from 29.6% to 84.5% among the 7 species using RAPD-data. A dendrogram for phylogenetic relationships separated the seven genotypes into two clusters. ISSRPCR technique using 10 primers showed a total number of 164 fragments across the seven genotypes. Fifty species-specific markers were detected. Similarity indices ranged between 27.4% and 69.6%. Dendrogram tree based on ISSR analysis showed two clusters, while, combined data of RAPD- and ISSR-PCR showed similarity indices ranged between 29.6% and 78.3 and a dendrogram tree with the same two clusters; one for Mentha species and the other for Ocimum species.

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