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Molecular genetics relationships among some bread wheat cultivars (Triticum aestivum L.) [electronic resource].

By:

Abo-Doma, A

Contributor(s):

Azzam, Clara R

Description: p.387-400Uniform titles:

Egyptian journal of genetics and cytology, 2007. v. 36 (2) [electronic resource].

Subject(s):

Online resources:

Full Text Article.

In: Egyptian Journal of Genetics and Cytology 2007.v.36(2)Summary: Twelve wheat cultivars namely, Sakha 61, Sakha 93, Sakha 94, Sakha 69, Sahel 1, Gemmieza 7, Gemmieza 9, Gemmieza 10 and Sids 1 (hexaploid) and Beni Suef 3, Beni Suef 1 and Sohag 3 were grown up to the seed maturity stage in a randomized complete block design with four replications during the two successive seasons of 2005-2006 and 2006-2007. Traits measured were plant height (cm), number of tillers/plant, number of leaves/plant, number of spikes/plant, weight of the main spike, number of seeds/spikes, shoot fresh weight and length of YEB leaf. The data obtained were statistically analyzed to determine the highest and the lowest cultivars in each of the measured traits. SSR-PCR analysis was performed using five primers to obtain markers to assist selection for some of the aforementioned traits in wheat. Primers Xg111 and Xg295 produced three SSR fragments with different lengths which could be considered cultivar specific markers for some of the yield related traits in wheat. ISSRPCR analysis was performed using ten primers to obtain markers to assist selection for some of the aforementioned traits in wheat. Primers 844A, 17889A, HB1, HB9, HB12 and HB15 produced ISSR fragments with different lengths could be used as markers to assist selection for some of the yield-related traits in wheat. Similarity index and the consensus tree were developed based on the ISSR-banding patterns of the twelve cultivars. The most two closely related cultivars were Sakha 94 and Gemmieza 10 with the highest similarity index (0.94) and the most two distantly related cultivars were Sakha 61 and Gemmieza 7 with low similarity index (0.58). The consensus tree was divided into two main clusters, the first included cultivars Sakha 61, Sakha 93 and Beni Suef 3. The second main cluster was divided into two subclusters. The first sub-cluster includes cultivars Sakha 94, Gemmieza 7, Gemmieza 9, Gemmieza 10 and Sahel 1 and the second one includes cultivars Beni Suef 1, Sids 1, Sohag 3 and Sakha 69.

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Twelve wheat cultivars namely, Sakha 61, Sakha 93, Sakha 94, Sakha 69, Sahel 1, Gemmieza 7, Gemmieza 9, Gemmieza 10 and Sids 1 (hexaploid) and Beni Suef 3, Beni Suef 1 and Sohag 3 were grown up to the seed maturity stage in a randomized complete block design with four replications during the two successive seasons of 2005-2006 and 2006-2007. Traits measured were plant height (cm), number of tillers/plant, number of leaves/plant, number of spikes/plant, weight of the main spike, number of seeds/spikes, shoot fresh weight and length of YEB leaf. The data obtained were statistically analyzed to determine the highest and the lowest cultivars in each of the measured traits. SSR-PCR analysis was performed using five primers to obtain markers to assist selection for some of the aforementioned traits in wheat. Primers Xg111 and Xg295 produced three SSR fragments with different lengths which could be considered cultivar specific markers for some of the yield related traits in wheat. ISSRPCR analysis was performed using ten primers to obtain markers to assist selection for some of the aforementioned traits in wheat. Primers 844A, 17889A, HB1, HB9, HB12 and HB15 produced ISSR fragments with different lengths could be used as markers to assist selection for some of the yield-related traits in wheat. Similarity index and the consensus tree were developed based on the ISSR-banding patterns of the twelve cultivars. The most two closely related cultivars were Sakha 94 and Gemmieza 10 with the highest similarity index (0.94) and the most two distantly related cultivars were Sakha 61 and Gemmieza 7 with low similarity index (0.58). The consensus tree was divided into two main clusters, the first included cultivars Sakha 61, Sakha 93 and Beni Suef 3. The second main cluster was divided into two subclusters. The first sub-cluster includes cultivars Sakha 94, Gemmieza 7, Gemmieza 9, Gemmieza 10 and Sahel 1 and the second one includes cultivars Beni Suef 1, Sids 1, Sohag 3 and Sakha 69.

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