Isolation and characterization of Cab8 gene from wild Vicia cinera species [electronic resource].

Includes references.

Vicia cinera is one of the common plants grown in the North West Coastal region of Egypt. Plants there are subjected to different abiotic stresses due to the harsh conditions. For that reason, these plants serve well to fish for stress related genes. Total RNA was isolated from Vicia cinera leaves and screened for the presence of Cab8 gene. Cab8 gene was successfully isolated, cloned, sequenced and submitted to gene bank with the accession no. AB505862 for DNA sequence and BAH70299.1 for expressed protein sequence. Taking in account that this is the first time to isolate the Cab8 gene from Vicia species, it showed 89% homology in the BLAST search with the Cab8 gene from Pisum sativum. The expression of the isolated sequence was tested in E. coli with an IPTG induction experiment. The isolated sequences were subjected to different computer analysis tools such as the comparative genome analysis and phylogenetic analysis in order to complete its characterization and see its relationship and ancestral origin to the other plant species. Even though the original organism where the Cab8 gene was isolated from, Vicia cinera, was not used in the comparative mapping, instead Pisum sativum data served well in the comparison to the legume model plant Medicago truncatula. The markers that were linked and mapped to Pisum sativum and Glycine max helped in the predictions, and their close proximity to our marker, the h2_20m4a (Cab8 gene) support the fact that it could be present near the CrS marker on chromosome 5 in the Pisum sativum, and near A235_1 marker on chromosome/clone G in Glycine max.

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