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Assessment of genetic variation and presence of nitrate reductase gene (NR) in different lettuce genotypes using PCR-based markers [electronic resource].

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AL-Redhaiman, K

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Language: English Summary language: Arabic Description: p.609-617Other title:

تحديد الاختلافات الوراثية والكشف عن نشاط جين النترات فى التراكيب الوراثية المختلفة للخس باستخدام الدلائل الجزيئية بواسطة الـ PCR [Added title page title]

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Annals of agricultural science, 2004 v. 49 (2) [electronic resource].

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In: Annals of Agricultural Science 2004.v.49(2)Summary: Thirteen lettuce genotypes belonging to four lettuce types (Butterhead, CosIRomaine, Crisphead, and Stem lettuce) were investigated using RAPD fingerprints as genetic marker to assess genetic diversity. Also, the presence of nitrate reductase gene (NR) in lettuce genotypes was investigated by PCR assays. From 30, random primers tested, only 13 were reproducible, giving 85 bands. The genetic similarity was estimated based on the pair-wise comparison of amplification products. The thirteen lettuce genotypes showed variation at the DNA level. The UPGMA cluster analysis separated the 13 lettuce genotypes into two distinct groups.

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Thirteen lettuce genotypes belonging to four lettuce types (Butterhead, CosIRomaine, Crisphead, and Stem lettuce) were investigated using RAPD fingerprints as genetic marker to assess genetic diversity. Also, the presence of nitrate reductase gene (NR) in lettuce genotypes was investigated by PCR assays. From 30, random primers tested, only 13 were reproducible, giving 85 bands. The genetic similarity was estimated based on the pair-wise comparison of amplification products. The thirteen lettuce genotypes showed variation at the DNA level. The UPGMA cluster analysis separated the 13 lettuce genotypes into two distinct groups.

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