Lectin histochemistry of the glandular part of the gastric mucosa of zebra (equus burchellii) [electronic resource].

By: Contributor(s): Description: p.85-103Uniform titles:
  • Journal of veterinary anatomy, 2009 v. 2 (1) [electronic resource].
Subject(s): Online resources: In: Journal of Veterinary Anatomy 2009.v2(1)Summary: The Fundic glands of the stomach of Zebra were histochemically analyzed by conventional and lectin histochemistry. The surface mucous cells (SMC) and foveolar mucous cells (FMC) were highly rich with glycoprotein (GPs) with oxidizable vicinal diols and/or glycogen and GPs with carboxyl groups and/ or GPs with O-sulphate esters. The GPs with oxidezable vicinal diols and/or glycogen were predominates in FMC while, the GPs with carboxyeI groups and/ or GPs with o-sulphate esters were predominate in SMC. The glandular epithelium of the base region of the fundic gland was devoid of both types of the GPs. All mucous secretig cells (SMC, FMC, mucous neck cells (MNC) and glandular mucous cells (GMS) were highly rich with a- anomer of the N-acetylgalacto-samine (aGaINAc), N- acetyl- glucosamine (GlcNAc) and neuroaminic acid (NeuNAc). The SMC and FMC were devoid of a- D-glucose (a- D- Glc), aD- mannose (a- D- Man), galac-Tose (Gal) and 13- anomer of the N¬acetylgalactosamine (13- GaINAc). The parietal cells (PC) appeared to react with Helix pomatia agglutinin (HPA), wheat germ agglutinine (WGA) and peanut agglutinin (PNA) indicating the existence of a- and 13- anomer of the GalNAc and NeuNAc. Key words Lectin, Histochemistry, Zebra, sugar residues.
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The Fundic glands of the stomach of Zebra were histochemically analyzed by conventional and lectin histochemistry. The surface mucous cells (SMC) and foveolar mucous cells (FMC) were highly rich with glycoprotein (GPs) with oxidizable vicinal diols and/or glycogen and GPs with carboxyl groups and/ or GPs with O-sulphate esters. The GPs with oxidezable vicinal diols and/or glycogen were predominates in FMC while, the GPs with carboxyeI groups and/ or GPs with o-sulphate esters were predominate in SMC. The glandular epithelium of the base region of the fundic gland was devoid of both types of the GPs. All mucous secretig cells (SMC, FMC, mucous neck cells (MNC) and glandular mucous cells (GMS) were highly rich with a- anomer of the N-acetylgalacto-samine (aGaINAc), N- acetyl- glucosamine (GlcNAc) and neuroaminic acid (NeuNAc). The SMC and FMC were devoid of a- D-glucose (a- D- Glc), aD- mannose (a- D- Man), galac-Tose (Gal) and 13- anomer of the N¬acetylgalactosamine (13- GaINAc). The parietal cells (PC) appeared to react with Helix pomatia agglutinin (HPA), wheat germ agglutinine (WGA) and peanut agglutinin (PNA) indicating the existence of a- and 13- anomer of the GalNAc and NeuNAc. Key words Lectin, Histochemistry, Zebra, sugar residues.

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