Karyotype and nucleic acids patterns of the camel nasal bot fly Cephalopina titillator (Clark) (Diptera: Oestridae) [electronic resource].

Includes references.

Studies on the chromosome number of the camel nasal bot fly, Cephalopina titillator proved the presence of five pairs of biarmed chromosomes (2n=10). Among the Calyptrata, the camel nasal bot fly is one of the few species that doesn't conform to the general rule of 2n=12. The designation of the chromosomes was characterized by the presence of three metacentric and two submetacentric pairs. No sign of heterogamety was found where the all five pairs were homomorphic. The haploid genome length measures was 25.09 f/m. The nucleic acids pattern was detected in different tissues (haemolymph, cuticle, fat body, Malpighian tubules and gut) of the 3rd larval instar of C. titillator. The results showed that each investigated tissue ofthe insect had DNA and RNA finger print or pattern that reflects the variations of gene expression from tissue to another. The comparison for the changes in DNA content in different tissues showed that the released DNA was separated into one band in all studied tissues. On the other hand, RNA was found to be separated into two main bands in cuticle, fat body, haemolymph and Malpighian tubules, whereas in the gut RNA was separated into three bands. The intensity of DNA and RNA in different tissues was measured as optical density. Malpighian tubules showed a drastic elevation in its nucleic acids intensity in respect to the other studied tissues. The electrophoretic pattern of DNA recorded its lowest level in haemolymph and gut. The electrophoretic pattern of RNA for Malpighian tubules, fat body and haemolymph detected a profile closely similar to that found with DNA

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