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Meiotic stages and incidence of diploid oocites in Egyptian cattle and buffaloes [electronic resource].

By:

Mahmoud, Karima Gh. M

Language: English Summary language: Arabic Description: p.336-347Other title:

الاطور الميوزية ونسبة البويضات ثنائية العدد الكروموسومى فى الابقار والجاموس المصرى [Added title page title]

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Assiut veterinary medical journal, 2004 v. 50 (102) [electronic resource].

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In: Assiut Veterinary Medical Journal 2004.v.50(102)Summary: The present work describes a cytogenetic study of in vitro matured oocytes from cattle and buffalo to analyze the meiotic stages,to determine the incidence of diploid metaphase II and to evaluate the in vitro maturation progress. Oocytes were collected by aspiration from ovaries of slaughtered cattle and buffalo.Recovered oocytes with at least two layers of compact cumulus cells and homogenous cytoplasm were selected. A total of 721 cattle and 989 buffalo oocytes were matured in droplets of TCM-199 supplemented with 10 % fetal calf serum and 50 J.lglml gentamycin at 39°C in 5 % C02 incubator for two periods 21-24 hrs and 25-28 hrs.

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The present work describes a cytogenetic study of in vitro matured oocytes from cattle and buffalo to analyze the meiotic stages,to determine the incidence of diploid metaphase II and to evaluate the in vitro maturation progress. Oocytes were collected by aspiration from ovaries of slaughtered cattle and buffalo.Recovered oocytes with at least two layers of compact cumulus cells and homogenous cytoplasm were selected. A total of 721 cattle and 989 buffalo oocytes were matured in droplets of TCM-199 supplemented with 10 % fetal calf serum and 50 J.lglml gentamycin at 39°C in 5 % C02 incubator for two periods 21-24 hrs and 25-28 hrs.

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