A study on in vitro propagation of pineapple (Ananas comosus l merr.) [electronic resource].

By: Contributor(s): Language: English Summary language: Arabic Description: p.371-389Other title:
  • إكثار الأناناس بواسطة زراعة الأنسجة.‪ [Added title page title]
Uniform titles:
  • Mansoura University journal of agricultural sciences, 2004 v. 29 (1) [electronic resource].
Subject(s): Online resources: In: Mansoura Unviersity Journal of Agricultural Sciences 2004.v.29(1)Summary: In several trails for surface sterilization by immersing the explants in Noacl (1 ,5,10.15 %) + (1.0 %) Hg CI2 for 10.20.30 minutes, the least contaminated explants were obtained by immersing the explants in NaOcl (15%) + (1.0%) HgCI2 for 30 minutes.During the establishment stage after 3 weeks the explants were cultured on liquid and solid MS media. full and half strength supplemented with BAP at 0 , 1 ,2 • 3 mgIL and IBA at 0 , 0.5 • 1.0 mglL for other 6 weeks. The best multiplication media was full strength liquid MS media supplemented with 1 mglL BAP + 1 mg/L IBA .In rooting stage , shootlet were cultured on media containing 0.5 x strength MS salts , 3 mgIL activated charcoal and combinations of IBA at (0 • 0.2 • 0.5 , 1.0 , 2.0 mgIL) and NAA at (0 • 0.2 , 0.5 , 1.0 mg/L) , the rooted shootlets were obtained by culturing on MS media supplemented with 1 mgIL NAA + 0.5 mg/L IBA .
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In several trails for surface sterilization by immersing the explants in Noacl (1 ,5,10.15 %) + (1.0 %) Hg CI2 for 10.20.30 minutes, the least contaminated explants were obtained by immersing the explants in NaOcl (15%) + (1.0%) HgCI2 for 30 minutes.During the establishment stage after 3 weeks the explants were cultured on liquid and solid MS media. full and half strength supplemented with BAP at 0 , 1 ,2 • 3 mgIL and IBA at 0 , 0.5 • 1.0 mglL for other 6 weeks. The best multiplication media was full strength liquid MS media supplemented with 1 mglL BAP + 1 mg/L IBA .In rooting stage , shootlet were cultured on media containing 0.5 x strength MS salts , 3 mgIL activated charcoal and combinations of IBA at (0 • 0.2 • 0.5 , 1.0 , 2.0 mgIL) and NAA at (0 • 0.2 , 0.5 , 1.0 mg/L) , the rooted shootlets were obtained by culturing on MS media supplemented with 1 mgIL NAA + 0.5 mg/L IBA .

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