Evaluation of genetic activity of some pesticides (Malathion, lannate) by using prophage induction and transduction analysis mechanisms [electronic resource].

Includes references.

The genetic activity of two pesticides have been evaluated using a battery of microbial tests. These tests were survival percentage, prophage induction and transduction analysis. The survival percentage was decreased by increasing the concentration of Malathion and Lannate up to 1600 ppm. The concentrations ranged from 100-800 ppm of malathion and 100-1000 ppm of lannate were found to have a mutagenic response when tested as inducing agents for F116 prophage from lysogenic strain. Transduction frequency was also varied with the different concentrations. Concentrations up to 1200 ppm of pesticides resulted in significant increase in number of transductants, and subsequently the transduction frequency was also increased. Transduction frequency at 600 ppm of malathion was 1.1: t 10.9 whereas at 800 ppm of lannate was 8.8 x 10-10. There was a good correlation between number of transductants, phage counts and phage induced. So the stimulation influence of both pesticides on transduction frequency rate may be due to their abilities as mutagenic agents to promote the SOS response that responsible for prophage induction mechanism. This may due to the increase in the number of transducing phage particles and hence resulting in increasing the rate of transduction.

Summary in Arabic.

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