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Genetic diversity among natural populations of drosophila based on RAPD markers [electronic resource].

By:

Mandour, A. S

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Language: English Summary language: Arabic Description: P. 1561-1578Other title:

التباعد الوراثى بين عشائر طبيعية من الدروسوفيلا بإستخدام واسمات الرابيد RAPD [Added title page title]

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Zagazig journal of agricultural research, 2004 v. 31 (4A) [electronic resource].

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Full Text Article.

In: Zagazig Journal of Agricultural Research 2004.v.31(4A)Summary: Random amplified polymorphic DNA (RAPD) analyses were performed by bulked genomic DNA extracted from four populations of Drosophila melanogaster and two populations of Drosophila, simulans with the objective of assessing genetic diversity among these populations. Samples of D. melanogaster populations were collected from the cultivated areas of Cairo, Sharkia, Ismailia and Menia governorates. Samples of D. simulans populations were collected from Alexandria and Ismailia governorates. Five out of 13 arbitrary decamer primers used in this study were informative and detected scoreable polymorphism in banding patterns of RAPD markers among the six Drosophila populations. Each of five primers used for analysis of individual Drosophila population amplified different number of bands. Genetic distances between Drosophila populations, calculated as the total number of band differences, were computed. The minimum distanceas found between Cairo and Menia populations, while the maximum distance was found between Sharkia population and Drosophila simulans population in Ismailia. The . dendrogram of genetic distances among populations of Drosophila based on band polymorphism generated by RAPD-PCR using all primers showed that the two Drosopl.ila simulans populations were separated in one cluster, the Menia and Cairo populations were grouped in the second cluster, while the populations of Sharkia and Ismailia (melanogaster) were found in the third cluster. Each individual population analyzed in this study had a unique genotype based on RAPD fingerprinting. The data indicated that it could be possible to differentiate DNA.

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Random amplified polymorphic DNA (RAPD) analyses were performed by bulked genomic DNA extracted from four populations of Drosophila melanogaster and two populations of Drosophila, simulans with the objective of assessing genetic diversity among these populations. Samples of D. melanogaster populations were collected from the cultivated areas of Cairo, Sharkia, Ismailia and Menia governorates. Samples of D. simulans populations were collected from Alexandria and Ismailia governorates. Five out of 13 arbitrary decamer primers used in this study were informative and detected scoreable polymorphism in banding patterns of RAPD markers among the six Drosophila populations. Each of five primers used for analysis of individual Drosophila population amplified different number of bands. Genetic distances between Drosophila populations, calculated as the total number of band differences, were computed. The minimum distanceas found between Cairo and Menia populations, while the maximum distance was found between Sharkia population and Drosophila simulans population in Ismailia. The . dendrogram of genetic distances among populations of Drosophila based on band polymorphism generated by RAPD-PCR using all primers showed that the two Drosopl.ila simulans populations were separated in one cluster, the Menia and Cairo populations were grouped in the second cluster, while the populations of Sharkia and Ismailia (melanogaster) were found in the third cluster. Each individual population analyzed in this study had a unique genotype based on RAPD fingerprinting. The data indicated that it could be possible to differentiate DNA.

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