Modulation of immunity and some biological functions of Japanese quail by Mannan oligosaccharide and B-Glucan administration [electronic resouce].

Includes references.

Three hundred sixty, 7 days old Japanese quail chick were randomly divided into six groups of 60 chicks, with similar initial average body weight, in three replicates of 20 birds each. Different levels from natural product of yeast cell walls' in form of (commercial suspension) containing 25 g/L MOS +30 g/L B-glucan were used in this experiment. The six treatment groups were used i.e negative and positive control (groups 1, 2) fed the basal diet, The other groups fed the basal diet and supplemented with different levels (0.5, 1, 1.5 and 2 milL respectively) of above suspension via drinking water from the second wk of age tell the end of experiment (six wk). All chicks from groups 2, 3, 4.5 and 6 were vaccinated against Newcastle Disease virus ( Lasota strain vaccine) at day 21 and repeated at day 28, respectively to evaluate the primary and secondary antibody responses. Five birds from each group at day 35 were injected in the right wing- web with 0.1 mL of phytohemagglutinin_ L. The swelling of the wings were measured before injection, at 24, 48 and 72 hrs, post injection, to measure the cell mediated immunity response. The results indicated that, the highest (P< 0.001) body weight gain was recorded for the Group fed on 0.5m11L (12.5 mg/L MOS +15 mg/L B-glucan) (group 3). A similar trend was nearly obtained for the feed conversion ratio (FCR) in which, the low level of MOS+B glucan significantly (P< 0.001) improved the feed efficiency as compared with the remaining treatments. Non Significant differences have been recorded in TP and Alb, but significant differences in Glb and AIG ratio were obtained compared with control chicb. Regarding to the total lipids, data clearly indicate significant decrease for chick fed (25 mg/L MOS + 30 mg IL B-glucan) (group 4) compared with the control and other treatments. Triglycerides as well as cholesterol levels diminished significantly due to application of low doses. AST activity was non significantly reduced in treated quails. The ~igh values of Lipase and amylase enzymes activities were found in (groups 3 and 4, respectively). The primary and secondary immune responses were improved for the chick fed low levels of MOS and B-glucan compared with other supplemented groups. It is clearly observed that, the control treatments(group 1, 2) and the high level of MOS + B glucan (group 6) had the lowest (P< 0.001) wing-web swelling at 24 h post PHA-L injection compared with the chick received other levels of MOS+B-glucan (group 3,4 and 5). Histological observation support the performance data, where the growth performance of the treatment groups was significantly improved It could be concluded that, treated Japanese quails with low levels of MOS and B-glucan improves growth performance, regulate lipid metabolism and enhance humoral and cellular immunity. Key Words: Immunity, Biological Functions, Oligosaccharkle, B-Glucan, Japanese Quail

Summary in Arabic.

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