Antiphytoviral activity of some natural substances on tobacco mosaic virus (menoufia strain) infecting tomato in Egypt and the physical properties of the viral inhibitors [electronic resource].

Includes reference.

Aqueous extracts of four plants were tested in vitro and in vivo for their antiviral properties against heat resistant strain (HRS) of tobacco mosaic virus (TMV). The effect of different dilutions of these extracts as well as the physical properties of the extract inhibitors was also studied. Obtained results showed that all the tested extracts had inhibitory effect on the virus, but in varying degrees. The inhibitory effect of the extracts increased with the increment of the storage period from 15 to 50 to 90 minutes both in vitro and in vivo. Using the in vitro technique showed higher inhibitory effect than that induced by in vivo. Pre-inoculation rubbing also showed more inhibition to virus infection than post inoculation rubbing. The most effective extract was that of Crocus sativus. The least effective one was that of Ocimum basilicum. Complete virus inhibition (100%) was obtained using the extract from C. sativus and Avena sativa. The antiviral activity of the used extracts was found to be highly affected by dilutions. The inhibitors were completely inactivated when kept at 40-85 °c for 10 minutes, survived for more than 85 days at room temperature and active at pH 2-11. The inhibitors in the extracts of C. sativus and A. sativa were not precipitated by a saturated solution of ammonium sulfate, while that of Tussilago farfara and o. basilicum were precipitated.

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