Role of the cultural conditions on the cbitinolytic activity of Trichoderma harzianum on Sckrotium rolfsii Mycelia [electronic resource].

Includes references.

The influence of environmental and cultural conditions was studied on the chilinolytic activity of T. harzianum on both chitin and mycelia of S. rolfsii (the causative agent of root rot of a wide range of hosts). The optimum temperature was 30.C, while the best pH-value was 5.0 after 5.0 days of incubation on either homogenized mycelia of S. rolfsii or pure chitin. The latter gave more chitinolytic activity in all the treatments. Among the tested carbon sources added beside chitin or the homogenized mycelia, 1.5% molass was the best. 1.0% peptone proved to be the most suitable nitrogen source for maximum chitinases activity. 1.5 and 2.0% of chitin or homogenized mycelia, respectively; gave the maximum values of chitinolytic activity by T. harzianum. The cultures of T. harzianum were formulated using soya bean flour as a carrier to biocontrol of the root rot disease of Phaseolus vulgaris. T. harzianum biomass (mycelia resulted after filtration of cultures) was more effective than the whole culture (biomass plus the culture filtrate) in decreasing the percentages of diseased plants compared with control values.

Summary in Arabic.

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