AFLP fingerprinting of some Egyptian date palm (Phoenix dactylifera L.) cultivars [electronic resource].

Includes references.

PCR-based DNA profiling of five Egvptian date palm (Phoenix dactylifera I-, cultivars was conducted using AFfP's. DNA samples from ten individual trees representing each or the .five cultivars were pooled to Jorm the sample representing the cultivar. A total of 433 amplificarion products were generated Jrom the .five cultivars using six primer pair combinations (EcoR1 and Msc1) with a mean, of 72.17 amplicons per assay. The information about genetic variation determined from AFLP data was employed to estimate genetic similaritv matrix value based on Jaccard's coefficient. The similarity values were Jurther used to construct a phonetic dendrogram revealing the genetic relationships. The dendrogram generated by the UPGMA (un-weighted pair group method using arithmetic averages) formed two major clusters with Siwi and Hayany being the most gelleticalll· similar cultivars, and in the second cluster Amhat and Samany being next. while Zaghloul was the most distinct cultivar. ALFP analysis also permitted the distinction of unique markers among the five studied date palm cultivars. A total of 78 positive and 48 negative markers were identified by the six AFLP primer combinations. The total number of unique markers per genotype ranged from 13 to 51. The cultivar Zaghloul was characterized by the highest number oj unique markers(51), while a total of 16, 23, 13 and 23 unique markers characterized lhe cultivars Siwi,Hayani Amhat, and Samany, respectively.

Summary in Arabic.

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