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Detection and characterization of virulence factors among Aeromonas species isolated from diseased fish [electronic resource].

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Hassan, Mohamed N

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Language: English Summary language: Arabic Description: P. 113-121Other title:

كشف و تمييز عوامل الضراوة في عترات الايروموناس المعزولة من الاسماك المريضة [Added title page title]

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Zagazig veterinary journal, 2011 v. 39 (2) [electronic resource].

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In: Zagazig Veterinary Journal 2011.v.39(2)Summary: Aeromonas species are distributed world-wide and infecting different aquatic organisms. Aeromonas are gram -ve, oxidase +ve, rods like bacteria which metabolize glucose by both oxidative and fermentative pathways. The aim of this investigation was to detect and characterize virulence factors among Aeromonas species from diseased Oreochromis niloticus. Two hundred of diseased fish were subjected to full clinical, post-mortem and bacteriological examinations. The total prevalence of Aeromonas spp. infection among the examined 0. niloticus was 65%. A. hydrophila was the highest incidence (40%) followed by A. sobria (17.5%) and A. caviae (7.5%). Nine Aeromonas isolates selected randomly (3 A. hydrophila, 3 A. sobria and 3 A. caviae) were tested for their virulence activity using the virulence tests as Congo red binding test, infant mice assay, verocells cytotoxicity assay and specific PCR tests for aerolysin and hemolysin genes detection.

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Aeromonas species are distributed world-wide and infecting different aquatic organisms. Aeromonas are gram -ve, oxidase +ve, rods like bacteria which metabolize glucose by both oxidative and fermentative pathways. The aim of this investigation was to detect and characterize virulence factors among Aeromonas species from diseased Oreochromis niloticus. Two hundred of diseased fish were subjected to full clinical, post-mortem and bacteriological examinations. The total prevalence of Aeromonas spp. infection among the examined 0. niloticus was 65%. A. hydrophila was the highest incidence (40%) followed by A. sobria (17.5%) and A. caviae (7.5%). Nine Aeromonas isolates selected randomly (3 A. hydrophila, 3 A. sobria and 3 A. caviae) were tested for their virulence activity using the virulence tests as Congo red binding test, infant mice assay, verocells cytotoxicity assay and specific PCR tests for aerolysin and hemolysin genes detection.

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